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A7721

Sigma-Aldrich

JumpStart™ Taq Antibody

Adds hot-start capabilities to any Taq DNA Polymerase

Synonym(s):

Hot start Taq antibody

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About This Item

MDL number:
MFCD02100063
UNSPSC Code:
41106300
NACRES:
NA.55

biological source

mouse

conjugate

unconjugated

clone

monoclonal

form

liquid

feature

hotstart

concentration

1.1 mg/mL

technique(s)

PCR: suitable

color

colorless

shipped in

wet ice

storage temp.

−20°C

Related Categories

General description

JumpStart™ Taq Antibody provides an efficient method for hot-start PCR. This antibody is a neutralizing monoclonal antibody specific to Taq DNA polymerase. When the antibody is bound to Taq, the enzyme is rendered inactive. At the first denaturation step in thermal cycling, the enzyme-antibody complex dissociates, and the JumpStart Taq Antibody becomes nonfunctional. Once the antibody is denatured, the activity of the Taq DNA polymerase is restored and the enzyme functions normally during the PCR reaction. JumpStart Taq Antibody is commonly used to prevent undesirable amplification products.

Application

JumpStart™Taq Antibody is suitable for use in:
  • cytochrome blocked nucleic acid (LNA) probe assay
  • ligation-mediated polymerase chain reaction (LM-PCR)
  • in Real-Time quantitative PCR Analysis of viral titer
  • PCR amplification of complex genomic or cDNA templates
  • to amplify very low-copy-number targets
  • for the PCR amplification of many thermal cycles (>35) and multiple primer pairs in the same reaction tube

Packaging

Supplied with dilution buffer.

Components

JumpStart Taq Antibody, 1.1μg/1μL
Dilution Buffer provided in 1 mL vials

Quantity

Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.

Other Notes

JumpStart™ Taq Antibody is for R&D use only.Not for drug, household, or other uses.

View more detailed information on JumpStart Taq enzymes at www.sigma-aldrich.com/hotstart.

Legal Information

JumpStart is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A7721-BULK:
A7721-200TST:
A7721-VAR:
A7721-500TST:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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D J Sharkey et al.
Bio/technology (Nature Publishing Company), 12(5), 506-509 (1994-05-01)
We demonstrate the utility of antibodies to the DNA polymerase from Thermus aquaticus (TaqPol) as thermolabile inhibitors of TaqPol activity. One of the limitations of the polymerase chain reaction (PCR) is the co-amplification of non-specific products caused by TaqPol activity
Enhanced gene targeting of adult and pluripotent stem cells using evolved adeno-associated virus
Gene correction, 169-179 (2014)
Measuring and monitoring apoptosis and drug toxicity in HIV patients by ligation-mediated polymerase chain reaction
Hooker DJ, et al.
Journal of Cellular and Molecular Medicine, 13(5), 948-958 (2009)
Application of real-time and multiplex polymerase chain reaction assays to study leaf blotch epidemics in barley
Fountaine JM, et al.
Phytopathology, 97(3), 297-303 (2007)

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