239764

Cycloheximide, High Purity

Antifungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes.

• Synonym(s): Cycloheximide, High Purity
• Empirical Formula (Hill Notation): C₁₅H₂₃NO₄
• CAS Number: 66-81-9
• Molecular Weight: 281.35
• MDL number: MFCD00082346
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥98% (HPLC)
• form: solid
• potency: 3.4 μM K_i
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: chloroform: soluble; ethanol: soluble; methanol: soluble
• shipped in: ambient
• storage temp.: 10-30°C
• InChI: 1S/C15H23NO4/c1-8-3-9(2)15(20)11(4-8)12(17)5-10-6-13(18)16-14(19)7-10/h8-12,17H,3-7H2,1-2H3,(H,16,18,19)/t8-,9-,11-,12+/m0/s1
• InChI key: YPHMISFOHDHNIV-FSZOTQKASA-N

Description

General description

Antifungal antibiotic that does not inhibit several species of pathogenic bacteria at 100 µg/ml. Inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFkBP12 (K_i = 3.4 µM). Triggers apoptosis in HL-60 cells, T-cell hybridomas, Burkitt′s lymphoma cells, and a variety of other cell types, including rodent macrophages. Inhibits DNA cleavage in rat thymocytes treated with thapsigargin, methylprednisolone, and ionomycin. Rapidly destroyed in alkaline solutions.

Antifungal antibiotic that inhibits protein synthesis in eukaryotes but not in prokaryotes. Interacts directly with the translocase enzyme, interfering with the translocation step. Inhibits cell-free protein synthesis in eukaryotes. Competitively inhibits hFKBP12 (K_i = 3.4 µM). Triggers apoptosis in HL-60 cells, T cell hybridomas, Burkitt’s lymphoma cells, and a variety of other cell types including rodent macrophages. However, it inhibits DNA cleavage in rat thymocytes treated with Thapsigargin (Cat. No. 586005), methylprednisolone, and Ionomycin (Cat. Nos. 407950 and 407952). Rapidly destroyed in alkaline solutions. Also available as a 300 mM solution in DMSO (Cat. No. 508739).

Biochem/physiol Actions

Primary Target
hFKBP12

Warning

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C, pH 3-5.

Other Notes

Christner, C., et al. 1999. J. Med. Chem. 42, 3615.
Lu, Q., et al. 1996. Arch. Biochem. Biophys.334, 175.
Chow, S.C., et al. 1995. Exp. Cell Res.216, 149.
Cotter, T.G., et al. 1992. Anticancer Res.12, 773.
Takano, Y.S., et al. 1991. J. Pathol.163, 329.
Waring, P. 1990. J. Biol. Chem. 265, 14476.

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H300,H341,H360D,H411
• Precautionary Statements: P202 - P264 - P273 - P280 - P301 + P310 - P391
• Hazard Classifications: Acute Tox. 2 Oral - Aquatic Chronic 2 - Muta. 2 - Repr. 1B
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable