for molecular biology
IR spectroscopy: suitable
0.08 mM (20-25°C)
DNase and RNase, none detected
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Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
10 - Combustible liquids
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Nonidet 40 or NP40 is no longer commercially available. The Igepal CA-630 (Product No. I8896), is the best substitute product that we can recommend.
Igepal CA-630 is chemically indistinguishable to NP40. This non-denaturing detergent is used the same way as that of NP40.
It is a 100% concentrated liquid product. It is not diluted. Please check the Specification sheet available on our product detail page.
The product I8896 is miscible in all proportions with water.
The formula weight of this product is 603.
The CMC value of this product is 0.083 mM.
The viscosity is determined for each lot and the information is available on the lot- specific Certificate of Analysis.
Molecular Biology Grade means that this product must meet the DNase, RNase, and Nickase specification on the Certificate of Analysis for each lot of the product.
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Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.