1.04220

Guanidinium chloride

LAB

• Synonym(s): Guanidine hydrochloride, Guanidinum hydrochloride, Carbamimidoylazanium chloride, Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidinium chloride
• Linear Formula: NH₂C(=NH)NH₂ · HCl
• CAS Number: 50-01-1
• Molecular Weight: 95.53
• Beilstein: 3591990
• MDL number: MFCD00013026
• EC Index Number: 200-002-3

Properties

• Quality Level: 100
• potency: 655.3-907.1 mg/kg LD₅₀, oral (Rat); >2000 mg/kg LD₅₀, skin (Rabbit)
• pH: 4.5-5.5 (20 °C, 100 g/L in H₂O)
• mp: 180-185 °C (lit.); 188 °C
• solubility: 2150 g/L
• density: 1.3 g/cm³ (lit.)
• bulk density: 550‑620 kg/m³
• storage temp.: 2-30°C
• SMILES string: Cl[H].NC(N)=N
• InChI: 1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H
• InChI key: PJJJBBJSCAKJQF-UHFFFAOYSA-N

Description

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Analysis Note

Assay (perchloric acid titration): ≥ 99.0 %
Identity (IR-spectrum): passes test
pH-value (10 %; water): 4.5 - 5.5
Melting range: 182 - 186
Chloride (calc. on anhydrous substance): 36.1 - 37.1 %

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302 + H332 - H315 - H319
• Precautionary Statements: P261 - P264 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable