Merck
All Photos(1)

1.04219

Millipore

Guanidinium chloride

for biochemistry

Synonym(s):
Aminoformamidine hydrochloride, Aminomethanamidine hydrochloride, Guanidine hydrochloride, Guanidinum hydrochloride, Carbamimidoylazanium chloride, Guanidinium chloride
Linear Formula:
NH2C(=NH)NH2 · HCl
CAS Number:
Molecular Weight:
95.53
Beilstein:
3591990
MDL number:
EC Index Number:
200-002-3

Quality Level

potency

655.3-907.1 mg/kg LD50, oral (Rat)
>2000 mg/kg LD50, skin (Rabbit)

pH

4.5-5.5 (20 °C, 100 g/L in H2O)

mp

180-185 °C (lit.)

density

1.3 g/cm3 (lit.)

bulk density

550‑620 kg/m3

storage temp.

2-30°C

SMILES string

Cl[H].NC(N)=N

InChI

1S/CH5N3.ClH/c2-1(3)4;/h(H5,2,3,4);1H

InChI key

PJJJBBJSCAKJQF-UHFFFAOYSA-N

General description

Biological detergent
Agent for reversible denaturation of proteins. Special grade with low optical density for use in procedures where spectrometry is involved.

Application

Strong chaotropic agent useful for the denaturation and subsequent refolding of proteins. This strong denaturant can solubilize insoluble or denatured proteins such as inclusion bodies. This can be used as the first step in refolding proteins or enzymes into their active form. Urea and dithiothreitol (DTT) may also be necessary.

Analysis Note

Assay (Perchloric acid titration, calc. on anhydrous substance): ≥ 99.5 %
Identity (IR-spectrum): passes test
pH (100 g/l, water): 5.5 - 6.5
Melting point: 183 - 188 °C
Absorbance A (235 nm; 50 %; 1 cm; water): ≤ 0,2
Heavy metals (as Pb): ≤ 10 ppm
Fe (Iron): ≤ 5 ppm
Sulfated ash (600 °C): ≤ 0.05 %
Water (according to Karl Fischer): ≤ 1

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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