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100600P

Avanti

E. coli Extract Polar

Avanti Research™ - A Croda Brand

Synonym(s):

E. coli Polar Lipid Extract

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About This Item

CAS Number:
1240502-50-4
UNSPSC Code:
12352211
NACRES:
NA.25

form

powder

packaging

pkg of 1 × 1 g (100600P-1g)
pkg of 1 × 100 mg (100600P-100mg)
pkg of 5 × 100 mg (100600P-500mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand

lipid type

lipid extracts

shipped in

dry ice

storage temp.

−20°C

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

Application

E. coli Extract Polar has been used for the preparation of lipid standards for validation purposes. It has also been used for determining the molar percentages of lipids.
E. coli Extract Polar has been used:
  • in liposome preparation for spectroscopy studies using fluorescent membrane dye, laurdan
  • in multilamellar vesicles generation
  • as standard lipid material for tandem mass spectrometry analysis

E. coli Extract Polar is suitable: to prepare lipid standard material by dissolving in methanol to prepare small unilamellar vesicle to prepare liposomes (in Tris-hydrochloric acid (HCl)

Biochem/physiol Actions

E. coli Polar lipid extracts are useful for reconstituting membrane proteins. The phosphatidylethanolamine is the predominant lipid component of E. coli extract polar extract. The minor components include the phosphatidylglycerol (PG) and cardiolipin. These lipids are majorly derived from the leaflets of cytoplasmic membrane and inner leaflet of outer membrane. E. coli polar lipid extracts form bilayer by vesicle fusion.

Packaging

5 mL Clear Glass Sealed Ampule (100600P-100mg)
5 mL Clear Glass Sealed Ampule (100600P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100600P-1g)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Storage Class Code

11 - Combustible Solids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

100600P-500MG:
100600P-VAR:
100600P-BULK:
100600P-1G:
100600P-100MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
Wenzel M, et al.
Bio-protocol, 8(20) (2018)
Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins
Methods in Cell Biology, 128, 223-241 (2015)
Molecular model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers
Scheidelaar S, et al.
Biophysical Journal, 108(2), 279-290 (2015)
Assessing membrane fluidity and visualizing fluid membrane domains in bacteria using fluorescent membrane dyes
Wenzel M, et al.
Bio-protocol (2018)
Masaru Sasaki et al.
The Journal of biological chemistry, 294(49), 18898-18908 (2019-10-31)
Bacterial membrane proteins are integrated into membranes through the concerted activities of a series of integration factors, including membrane protein integrase (MPIase). However, how MPIase activity is complemented by other integration factors during membrane protein integration is incompletely understood. Here
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