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100500P

Avanti

E. coli Extract Total

Avanti Research™ - A Croda Brand

Synonym(s):

E. coli Total Lipid Extract

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About This Item

CAS Number:
1240502-50-4
UNSPSC Code:
12352211
NACRES:
NA.25

form

powder

packaging

pkg of 1 × 1 g (100500P-1g)
pkg of 1 × 100 mg (100500P-100mg)
pkg of 5 × 100 mg (100500P-500mg)

manufacturer/tradename

Avanti Research™ - A Croda Brand

lipid type

lipid extracts

shipped in

dry ice

storage temp.

−20°C

General description

This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated.

Application

E. coli Extract Total has been used in the preparation of unilamellar vesicles in lipid screening experiments of membrane protein green fluorescent protein (GFP) based fusion proteins and in the reconstitution of proteoliposomes.

Biochem/physiol Actions

The E. coli lipid extract is a combination of 16, 17 and 18 carbon containing phospholipids namely, phosphatidylethanolamine, phosphatidylglycerol and cardiolipin. It is used for preparing supported lipid bilayers. The E. coli lipid extract serves as a standard for profiling phospholipids, aids in identification and taxonomic grouping of any bacterial sample.

Packaging

5 mL Clear Glass Sealed Ampule (100500P-100mg)
5 mL Clear Glass Sealed Ampule (100500P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100500P-1g)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

also commonly purchased with this product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

100500P-100MG:
100500P-BULK:
100500P-1G:
100500P-VAR:
100500P-500MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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An engineered thermal-shift screen reveals specific lipid preferences of eukaryotic and prokaryotic membrane proteins
Nji E, et al.
Nature Communications, 9(1), 4253-4253 (2018)
The extracellular gate shapes the energy profile of an ABC exporter
Hutter CAJ, et al.
Nature Communications, 10(1), 2260-2260 (2019)
Membrane insertion of small proteins
Protein Secretion, 39-62 (2010)
Sophie J Hesketh et al.
Biochimica et biophysica acta. Biomembranes, 1862(5), 183192-183192 (2020-01-17)
Membrane proteins are traditionally extracted and purified in detergent for biochemical and structural characterisation. This process is often costly and laborious, and the stripping away of potentially stabilising lipids from the membrane protein of interest can have detrimental effects on
Lipid compositions in Escherichia coli and Bacillus subtilis during growth as determined by MALDI-TOF and TOF/TOF mass spectrometry
Gidden J, et al.
International Journal of Mass Spectrometry, 283(1-3), 178-184 (2009)
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