HPLC Analysis of Proteins on Discovery® BIO PolyMA-WAX (Analyte Set #1)
Materiali
Standard
N° Catalogo
Descrizione
Determinazione del prezzo
Trypsin inhibitor from chicken egg white
Type II-O, Partially purified ovomucoid, containing ovoinhibitorCONDITIONS
column
Discovery BIO PolyMA-WAX, 5 cm x 4.6 mm, 5 μm particles (59602-U)
mobile phase
[A] 10 mM Tris, pH 8.0 with HCl; [B] 10 mM Tris, 0.5 M NaCl, pH 8.0 with HCl; 5 to 100% B in 15 min. (linear)
flow rate
0.5 mL/min
detector
UV, 280 nm
injection
10 μg trypsin inhibitor, 5 μg conalbumin, myoglobin or 10 μg ribonuclease A, 5 μg cytochrome c, a-chymotrypsinogen A
Descrizione
Risultati analitici
The large pore diameter of Discovery BIO PolyMA-SCX and PolyMA-WAX particles allows full access to small peptides and very large proteins and protein aggregates. This Figure shows the separation of proteins that vary from 18 to 80 kDA.
Note legali
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany