T9378

Trypsin inhibitor

crude powder

• Sinonimo/i: Trypsin Inhibitor from lima beans
• Numero CAS: 9035-81-8
• Numero CE: 232-906-9
• Numero MDL: MFCD00082102
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• product name: Trypsin inhibitor from Phaseolus limensis (lima bean), Type II-L, crude powder
• Origine biologica: Phaseolus limensis (lima bean)
• Livello qualitativo: 200
• Tipo: Type II-L
• Forma fisica: crude powder
• PM: 9 kDa
• Solubilità: 0.067 M sodium phosphate buffer, pH 7.6: 1 mg/mL
• Temperatura di conservazione: 2-8°C

Descrizione

Applicazioni

Trypsin inhibitor from Phaseolus limensis (lima bean) has been used:

• in the inhibition of trypsin in human plasma
• in the termination of proteolytic digestion in rabbit skeletal muscles
• in the inhibition of salivary glands enzyme extract from Lygus lineolaris
• in the inhibition of proteolytic activity in Agave tequilana enzyme extract

Trypsin inhibitor has been used as an affinity ligand for isolation of elastase-type enzymes.

Azioni biochim/fisiol

Trypsin inhibitor from lima beans belongs to Bowman-Birk family of protease inhibitors. It is 9 kDa in molecular weight and contains nine disulfide bridges and is highly thermostable. It has trypsin and chymotrypsin binding subdomains.

Proprietà fisiche

Monomer has an apparent molecular weight of approx. 9,000 Da but undergoes a concentration and pH-dependent dimerization.

Definizione di unità

One trypsin unit will produce a ΔA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 mL, 1 cm light path.

Nota sulla preparazione

Prepared by a modification of the method of Tauber, H., et al., J. Biol. Chem., 179, 1155 (1949) (modified).

Risultati analitici

One mg will inhibit ≥0.8 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Altre note

View more information on Trypsin Inhibitor.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H317,H334
• Consigli di prudenza: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Classi di pericolo: Resp. Sens. 1 - Skin Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)