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Key Documents

T2327

Sigma-Aldrich

Trypsin inhibitor

lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

Sinonimo/i:

Kunitz Inhibitor

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About This Item

Numero CAS:
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.77

product name

Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)

Origine biologica

Glycine max (soybean)

Livello qualitativo

Nome Commerciale

BioUltra

Saggio

≥95% (Kunitz inhibitor, SDS-PAGE)

Forma fisica

lyophilized powder

Temperatura di conservazione

2-8°C

Descrizione generale

Trypsin Inhibitor from Glycine max (soybean) also known as Kunitz trypsin inhibitor is a 21 kDa protein with a single trypsin binding reactive site.

Applicazioni

Trypsin Inhibitor from Glycine max (soybean) has been used:
  • as a standard protein to measure the amount of endogenous trypsin inhibitor present in midgut lysate (M1) of Riptortus pedestris
  • as a standard to compare the trypsin inhibitory activity of the purified protein
  • to monitor the trypsin inhibitory activity by fractionating in MonoS cation exchange chromatography
  • as an trypsin inhibitor

Azioni biochim/fisiol

Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. It forms a 1:1 stoichiometric complex with trypsin. Upon formation of this complex, trypsin may cleave a single arginine-isoleucine bond in the inhibitor. Dissociation of this complex may yield the modified form or the native inhibitor. At the optimal pH for trypsin binding (pH 8.0), the association constant is ≥ 10x108.
Trypsin Inhibitor from Glycine max (soybean) binds with the active site of trypsin enzyme, in a competitive inhibition manner.

Definizione di unità

One trypsin unit will produce a ΔA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

Nota sulla preparazione

Further purification of T9128 yielding an electrophoretically pure Kunitz inhibitor with increased activity.
Trypsin inhibitor is soluble in water and phosphate buffers at concentrations of 10 mg/ml or higher. Solutions at higher concentrations may be hazy and have a yellow to amber color.

Risultati analitici

One mg will inhibit ≥1.0 mg of trypsin with activity of approx. 10,000 BAEE units per mg protein.

Altre note

View more information on Trypsin Inhibitor.

Applicazioni

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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A midgut lysate of the Riptortus pedestris has antibacterial activity against LPS O-antigen-deficient Burkholderia mutants
Am Jang H, et al.
Developmental and Comparative Immunology, 67, 97-106 (2017)
Xingfei Li et al.
Journal of agricultural and food chemistry, 66(17), 4439-4448 (2018-03-23)
We first observed that protein/polysaccharide interaction exhibited noninteracting behavior which makes Bowman-Birk chymotrypsin inhibitor (BBI) always free of complexation, being separated from another protein with similar isoelectric points, Kunitz trypsin inhibitor (KTI). Turbidity titrations showed that the electrostatic attractions were
Functional analysis of the Kunitz trypsin inhibitor family in poplar reveals biochemical diversity and multiplicity in defense against herbivores
Major IT and Constabel CP
Plant Physiology, 146(3), 888-903 (2008)
A continuous fluorometric assay for trypsin based on melittin and the noncovalent-binding-induced pyrene excimer
Xu N, et al.
Chemistry Letters (Jpn), 42(12), 1528-1530 (2013)
Quantitative determination of active Bowman-Birk isoinhibitors, IBB1 and IBBD2, in commercial soymilks
Arques MC, et al.
Food Chemistry, 155, 24-30 (2014)

Protocolli

Enzymatic Assay of Trypsin Inhibitor

Chromatograms

application for HPLC

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