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HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18

HPLC Analysis of Aminopterin and Related Compounds on Ascentis® Express C18 application for HPLC

Materiali

Colonna analitica

N° Catalogo
Descrizione
Determinazione del prezzo

53829-U

Colonna per HPLC Ascentis® Express C18, 2,7 μm

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Standard

N° Catalogo
Descrizione
Determinazione del prezzo

F7876

Folic acid

≥97%

M9929

Metotrexato

meets USP testing specifications

CONDITIONS

column

Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] 10 mM ammonium formate, pH 3.0; [B] acetonitrile; (90:10, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 280 nm

injection

5 μL

Descrizione

Risultati analitici

This application demonstrates the suitability of the Ascentis C18 for the analysis of aminopterin, folic acid and methotrexate. Structures along with the optimized chromatogram obtained on the Ascentis Express C18 are presented

Note legali

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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