T7254

N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride

powder, ≥96% (TLC)

• Sinonimo/i: (3S)-1-Chloro-3-tosylamido-7-amino-2-heptanone hydrochloride, (3S)-7-Amino-1-chloro-3-tosylamino-2-heptanone hydrochloride, TLCK, Tosyl-L-lysyl-chloromethane hydrochloride
• Formula empirica (notazione di Hill): C₁₄H₂₁ClN₂O₃S · HCl
• Numero CAS: 4272-74-6
• Peso molecolare: 369.31
• Beilstein: 7106867
• Numero CE: 224-266-4
• Numero MDL: MFCD00065395
• Codice UNSPSC: 12352202
• ID PubChem: 24278752
• NACRES: NA.77

Proprietà

• Nome del prodotto: N_α-Tosyl-L-lysine chloromethyl ketone hydrochloride, ≥96% (TLC), powder
• Origine biologica: synthetic (organic)
• Livello qualitativo: 200
• Saggio: ≥96% (TLC)
• Stato: powder
• Potenza: 10-100 μM effective concentration
• Solubilità: H₂O: 50 mg/mL
• Temperatura di conservazione: −20°C
• Stringa SMILE: Cl[H].Cc1ccc(cc1)S(=O)(=O)N[C@@H](CCCCN)C(=O)CCl
• InChI: 1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16;/h5-8,13,17H,2-4,9-10,16H2,1H3;1H/t13-;/m0./s1; YFCUZWYIPBUQBD-ZOWNYOTGSA-N
• Informazioni sul gene: human ... NFKB1(4790) , NFKB2(4791)

Descrizione

Applicazioni

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride has been used:

• in chymotrypsin purification to prevent binding of trypsins to the affinity support by inhibiting them in the crude extract
• as a protease inhibitor in tissue and cell homogenization
• as a trypsin inhibitor to determine the specific protease activity levels

Azioni biochim/fisiol

Nα-Tosyl-L-lysine chloromethyl ketone hydrochloride (TLCK) blocks the lipopolysaccharide (LPS)- or cytokine-induced activation of nuclear factor κB (NF-κB), which, in turn, blocks the induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) transcription. Blocks activation of pp70s6k by all mitogens. Blocks apoptosis in cell lines by inhibiting the processing of caspases in some cell lines and to some stimuli.

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

Avvertenza

Stable at least two years when stored desiccated at −20°C.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: dust mask type N95 (US), Eyeshields, Gloves