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B4500

N_α-Benzoyl-L-arginine ethyl ester hydrochloride

Name: <I>N</I><SUB>α</SUB>-Benzoyl-<SC>L</SC>-arginine ethyl ester hydrochloride
Brand: SIGMA

trypsin substrate, chromogenic, ≥97% (HPLC), powder

Sinonimo/i:

BAEE

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About This Item

Formula empirica (notazione di Hill):

C₁₅H₂₂N₄O₃ · HCl

Numero CAS:

2645-08-1

Peso molecolare:

342.82

Beilstein:

3781694

Numero CE:

220-157-0

Numero MDL:

MFCD00012579

Codice UNSPSC:

12352204

ID PubChem:

24891777

NACRES:

NA.32

product name

N_α-Benzoyl-L-arginine ethyl ester hydrochloride, trypsin substrate

Saggio

≥97% (HPLC)

Forma fisica

powder

Solubilità

water: 50 mg/mL, clear, colorless

Temperatura di conservazione

2-8°C

Stringa SMILE

Cl[H].CCOC(=O)[C@H](CCCNC(N)=N)NC(=O)c1ccccc1

InChI

1S/C15H22N4O3.ClH/c1-2-22-14(21)12(9-6-10-18-15(16)17)19-13(20)11-7-4-3-5-8-11;/h3-5,7-8,12H,2,6,9-10H2,1H3,(H,19,20)(H4,16,17,18);1H/t12-;/m0./s1
HIXDELXKSSLIKB-YDALLXLXSA-N

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Descrizione generale

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.

Applicazioni

α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
for assaying proteolytic activity of enzymatic extracts of papaya plant parts
in trypsin activity assay of the tail tendon fascicles from rat

Substrati

The prototype substrate for trypsin.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Analytical chemistry, 77(14), 4604-4610 (2005-07-15)

Trypsin is covalently linked to a photopolymerized sol-gel monolith modified by incorporating poly(ethylene glycol) (PSG-PEG) for on-column digestion of N(alpha)-benzoyl-l-arginine ethyl ester (BAEE) and two peptides, neurotensin and insulin chain B. The coupling of the enzyme to the monolith is

The mechanical, structural, and compositional changes of tendon exposed to elastase

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Annals of Biomedical Engineering, 43(10), 2477-2486 (2015)

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Articoli

Papain

Papain is a cysteine protease of the peptidase C1 family. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.

Protocolli

Enzymatic Assay of Trypsin Inhibitor

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for products with a specification for Trypsin activity using Na-Benzoyl-L-arginine ethyl ester (BAEE) as a substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light path = 1 cm).

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