T4019
Trypsin−Agarose
buffered aqueous suspension, from bovine pancreas trypsin
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About This Item
Prodotti consigliati
Origine biologica
bovine pancreas (trypsin)
Livello qualitativo
Forma fisica
buffered aqueous suspension
Concentrazione
≥15 units/mL (packed gel)
Grado di funzionalizzazione
≥15 units per mL (packed gel)
Matrice
beaded agarose
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.
Applicazioni
An experimental approach using SDS-PAGE, in-gel trypsin digestion and proteomic analysis identified five endosomal proteins. Trypsin cleavage of the high-risk HPV-16 E5 protein (16E5) generates a unique four-amino acid C-terminal peptide that serves as a marker for E5 expression in transfected cells and epithelial cell lines containing integrated and episomal HPV-16 DNA.
Definizione di unità
One unit will hydrolyze 1.0 μmole of BAEE per min at pH 8.0 at 30 °C (titrimetric assay).
Stato fisico
Suspension in approx. 10 mM acetic acid, pH 3.2, containing preservative
Nota sulla preparazione
TPCK-treated
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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