SRP0721

AKT1 (E17K), Active

PRECISIO^® Kinase, recombinant

• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in baculovirus infected Sf9 cells
• Saggio: 70-95% (SDS-PAGE)
• Stato: frozen liquid
• Attività specifica: 50-75 nmol/min·mg (radiometric)
• PM: ~85 kDa
• Concentrazione: 0.1 μg/μL
• Numero d’accesso: NM_005163
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... AKT1(207)

Descrizione

Applicazioni

Useful for kinase assay.

Azioni biochim/fisiol

Recombinant full-length human AKT1 (E17K) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. AKT1/PKBalpha is a serine/threonine kinase that belongs to the AKT family. AKT1 is activated in cells in response to diverse stimuli such as hormones, growth factors and extracellular matrix components and is involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, and cell motility ⁽¹⁾. AKT1 is frequently overexpressed and active in many types of human cancers including cancers of colon, breast, brain, pancreas and prostate as well as lymphomas and leukemias ⁽²⁾.

Ricostituzione

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Stoccaggio e stabilità

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable