SRP0708

BTK (C481S), Active

PRECISIO^® Kinase, recombinant

• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Ricombinante: expressed in baculovirus infected Sf9 cells
• Saggio: 70-95% (SDS-PAGE)
• Forma fisica: frozen liquid
• Attività specifica: 40-56 nmol/min·mg (radiometric)
• PM: ~75 kDa
• Concentrazione: 0.1 μg/μL
• Numero d’accesso: NM_005228
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C

Descrizione

Applicazioni

Useful for kinase assay and Western blot.

Azioni biochim/fisiol

Recombinant full-length human BTK (C481S) was expressed by Baculovirus in Sf9 insect cells using an N-terminal His tag. BTK (also known as Bruton tyrosine kinase) plays a crucial role in B-lymphocyte differentiation and activation. BTK interacts with SRC homology 3 domains of FYN, LYN and HCK that are activated upon stimulation of B- and T-cell receptors ⁽¹⁾.Defects in the BTK gene cause Agammaglobulinemia, an X-linked immunodeficiency characterized by failure to produce mature B lymphocyte cells and associated with a failure of Ig heavy chain rearrangement. The unique role of BTK makes it a desirable target for potential anti-cancer, anti-inflammatory and anti-viral agents as well as other treatments ⁽²⁾.

Ricostituzione

Formulated in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, with 25% glycerol.

Stoccaggio e stabilità

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Pittogrammi: GHS08,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H315,H319,H360D
• Consigli di prudenza: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable