SRP0194

PARP3 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Sinonimo/i: ADPRT3, ADPRTL2, IRT1, NAD(+) ADP-ribosyltransferase 3, Poly (ADP-ribose) Polymerase 3
• Codice UNSPSC: 12352200
• NACRES: NA.77

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥80% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 87 kDa
• Confezionamento: pkg of 10 μg
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_001003931
• N° accesso UniProt: Q9Y6F1
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... PARP3(10039)

Descrizione

Descrizione generale

Human PARP3 (GenBank Accession No. NM_001003931), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definizione di unità

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.