SRE0021

Granzyme B human

recombinant, expressed in insect cells, ≥90% (SDS-PAGE)

• Sinonimo/i: Granzyme B human, Cytotoxic T-lymphocyte proteinase 2, Granzyme-2, GranzymeB, Lymphocyte protease, SECT, T-cell serine protease 1-3E
• Codice UNSPSC: 12352204
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in insect cells
• Saggio: ≥90% (SDS-PAGE)
• Forma fisica: lyophilized powder
• Potenza: >0.004 units/mg
• Attività specifica: ≥40 units/μg
• Confezionamento: pkg of 5 μg
• N° accesso UniProt: P10144
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... GZMB(3002)

Descrizione

Descrizione generale

Granzyme B (GzmB) is a serine protease which is stored in granules of activated cytotoxic T cells and NK cells. GZMB codes for granzyme B.

Azioni biochim/fisiol

Granzyme B is considered as the marker of activated CTLs (cytotoxic T-cells), which mediates target-cell apoptosis. It induces apoptosis in caspase-dependent and caspase-independent manner. After contact with target cells, granzyme B is directionally exocytosed, and then enters the target cell with assistance from perforin. Granzyme B specifically cleaves after Asp residues, and particularly processes and activates various pro-caspases. This induces apoptosis in the target cell.

Definizione di unità

One unit is defined as the amount of enzyme that hydrolyzes 1 pmol/min of IETD-AFC per min at 37°C in 50 mM HEPES, pH 7.5 containing 10 mM CaCl₂.

Stato fisico

Lyophilized powder, containing HEPES buffer (original solution at pH 7.5)

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable