SAB4200468

Anti-JAM-A antibody, Mouse monoclonal

clone J10.4, purified from hybridoma cell culture

• Sinonimo/i: Monoclonal Anti-CD321, Monoclonal Anti-F11 receptor, Monoclonal Anti-F11R, Monoclonal Anti-JAM, Monoclonal Anti-JAM1, Monoclonal Anti-JAMA, Monoclonal Anti-JCAM, Monoclonal Anti-KAT, Monoclonal Anti-PAM-1
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: J10.4, monoclonal
• Forma fisica: buffered aqueous solution
• PM: antigen 37 kDa
• Reattività contro le specie: human
• Concentrazione: ~1.0 mg/mL
• tecniche: immunoblotting: 1.0-2.0 μg/mL using HepG2 or A431 or A549 total cell extracts
• Isotipo: IgG1
• N° accesso UniProt: Q9Y624
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... F11R(50848)

Descrizione

Descrizione generale

Anti-JAM-A antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM. Junctional adhesion molecule A (JAM-A) is expressed at tight junctions of endothelial and epithelial cells as well as on a variety of hematopoietic cells. It belongs to the transmembrane IgG glycoprotein family and is also expressed in monocytes, lymphocytes, neutrophils, dendritic cells, platelets, and spermatozoa. The JAM-A or F11R gene is mapped to human chromosome 1q23.3.

Specificità

Anti-JAM-A antibody, Mouse monoclonal recognizes human JAM-A.

Immunogeno

derived from the hybridoma J10.4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant human JAM fusion protein.

Applicazioni

Anti-JAM-A antibody, Mouse monoclonal has been used in:

• western blotting
• staining for confocal microscopy
• immunoprecipitation
• immunocytochemistry
• flow cytometry

Azioni biochim/fisiol

Junctional adhesion molecule A(JAM-A) function has been linked with regulation of cell migration/invasion, platelet adhesion, cell polarity, cell proliferation, and epithelial or endothelial barrier function. However, the mechanisms by which JAM-A mediates these processes have not been well elucidated. JAM-A functional significance is connected with inflammation, angiogenesis, hypertension, ischemia/reperfusion, and atherosclerosis. JAM-A has also been linked to breast, endometrial, renal cell carcinomas and gastric cancer.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For extended storage, freeze at –20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable