SAB4200053

Anti-DNA polymerase δ p125 antibody, Rat monoclonal

clone PDG 5G1, purified from hybridoma cell culture

• Sinonimo/i: Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rat
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: PDG 5G1, monoclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~125 kDa
• Reattività contro le specie: human, mouse, rat
• Confezionamento: antibody small pack of 25 μL
• tecniche: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using Jurkat cells extract
• N° accesso UniProt: P28340
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... POLD1(5424)

Descrizione

Descrizione generale

Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.

Immunogeno

human p125

Applicazioni

Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• co-immunoprecipitation

Azioni biochim/fisiol

The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.

Stato fisico

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable