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S5897

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

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About This Item

Numero CAS:
9004-54-0
Numero MDL:
MFCD00132771
Codice UNSPSC:
23151817
NACRES:
NA.52

Grado

DNA grade
for molecular biology

Livello qualitativo

200

Nome Commerciale

BioReagent

Forma fisica

beads

tecniche

DNA purification: suitable

Matrice

cross-linked dextran

Rigonfiamento

1 g swells to 9-11 mL

Diametro delle microsfere

20-80 μm

Dimensione pori

exclusion limit (20 bp dsDNA (rA)20)

pH

2-10

Attività estranea

DNase, RNase, none detected

Temperatura di conservazione

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2
FZWBNHMXJMCXLU-UHFFFAOYSA-N

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Descrizione generale

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Applicazioni

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Caratteristiche e vantaggi

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Note legali

Sephadex is a registered trademark of Cytiva

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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