R0280

Anti-RanGAP1

affinity isolated antibody, buffered aqueous solution, ~2 mg/mL

• Sinonimo/i: Anti-Ran-GTPase-Activating Protein 1
• Numero MDL: MFCD09265372
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~65 kDa
• Reattività contro le specie: human
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• Concentrazione: ~2 mg/mL
• tecniche: indirect immunofluorescence: 10-20 μg/mL using HeLa cells; western blot: 1-2 μg/mL using HEK-293T cells transfected with human RanGAP1
• N° accesso UniProt: P46060
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... RANGAP1(5905) ; mouse ... Rangap1(19387)

Descrizione

Descrizione generale

Ran GTPase−activating protein 1 (RanGAP1), a guanosine-5′-triphosphatase (GTPase)-activating protein for Ran exists in two different types, where one is cytoplasmic and other is concerted at the cytoplasmic fibers of nuclear pore complexes (NPCs). Mammalian RanGAP1 has a C-terminal extension. Ran GTPase gene is located on human chromosome 22q13.2.

Specificità

Anti-RanGAP1 (N-terminal) recognizes human RanGAP1.

Immunogeno

synthetic peptide corresponding to amino acids 1-20 located at the N-terminus of human RanGAP1, conjugated to KLH. This sequence is identical in mouse RanGAP1.

Applicazioni

Anti-RanGAP1 (N-terminal) antibody produced in rabbit may be used immunoblotting and immunofluorescence.

Azioni biochim/fisiol

Ran GTPase−activating protein 1 (RanGAP1) regulates Ran activity. RanGAP1 can stimulate the GTPase activity of Ran. Ran is also regulated by a chromatin-bound nucleotide exchange factor, regulator of chromosome condensation 1 (RCC1) that keeps Ran in the active guanosine-5′-triphosphate (GTP)-bound state. RanGAP1 has the ability to conjugate to the small ubiquitin-related modifier protein small ubiquitin-related modifier 1 (SUMO-1). RanGAP1 may participate in nuclear protein import.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)