N9284
Nitroreductase from Escherichia coli
≥90% (SDS-PAGE), recombinant, expressed in E. coli
Sinonimo/i:
NTRA
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About This Item
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Ricombinante
expressed in E. coli
Livello qualitativo
Saggio
≥90% (SDS-PAGE)
Forma fisica
lyophilized powder
Attività specifica
≥100 units/mL
PM
monomer 24000
Caratteristiche più verdi
Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
N° accesso UniProt
Categoria alternativa più verde
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Informazioni sul gene
Escherichia coli K12 ... nfsB(945483)
Descrizione generale
Nitroreductase is a flavoprotein and is encoded by the NfsB gene. It comprises a dimer, with 217 amino acids and active site in each subunit. The structure has FMN and the substrate bound to the enzyme.
Applicazioni
Nitroreductase from Escherichia coli has been used in the conjugation generation with pig liver esterase (PLE). It has also been used in chemiluminescence response studies with probes HyCL-3 and HyCL-4-AM in rat liver microsomes.
Azioni biochim/fisiol
Nitroreductase (NTR) catalyzes the reduction of nitroaromatic substrates and quinones. The mutant F124K of NTR is useful in cancer therapy and improves sensitization of drug CB1954.
Nitroreductase increases the sensitivity of organisms to nitro-containing drugs such as metronidazole by converting the nitro group to a cytotoxic nitro radical.
Nitroreductases can play a crucial role in redox systems via NADPH or NADH as a reductant.
Shows ability to reduce quinines. Enzyme for activating prodrugs in antibody directed enzyme prodrug therapy.
Proprietà fisiche
Lyophilized powder containing PBS. Does not contain BSA as excipient
Definizione di unità
One unit will reduce one μmole of Cytochrome C per minute in the presence of Menadione and NADH at pH 7.4 at 37 °C.
Nota sulla preparazione
Produced using animal component-free materials.
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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