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MAK087

Sigma-Aldrich

Acid Phosphatase Activity Fluorometric Assay Kit

sufficient for 500 fluorometric tests

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Numero CE:
Codice UNSPSC:
12352200

impiego

sufficient for 500 fluorometric tests

Metodo di rivelazione

fluorometric

Temperatura di conservazione

−20°C

Informazioni sul gene

Descrizione generale

The new Acid Phosphatase Fluorometric Assay Kit, MAK525, is now available! Acid phosphatases (AP) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimal pH of 4-7. Changes in the levels and activities of AP occur in many pathophysiological conditions including prostate cancer and bone disease.

Caratteristiche e vantaggi

Compatible with high-throughput handling systems.

Compatibilità

Suitable for the detection of acid phosphatase in serum and other biological samples.

Principio

The AP Activity Fluorometric Assay Kit provides a simple and direct procedure for measuring AP activity in serum and other samples. AP activity is determined by the conversion of the non-fluorescent 4-methylumbelliferyl phosphate disodium salt (MUP) to the fluorescent product 4-methylumbelliferone (4-MU) (λex = 360/λem = 440 nm), proportional to the AP activity present. One unit of AP is the amount of enzyme that will hydrolyze 1.0 μmole of MUP per minute at 25 °C.

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Codice della classe di stoccaggio

12 - Non Combustible Liquids


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Significance of increased phosphatase activity of bone at the site of osteoplastic metastases secondary to carcinoma of the prostate gland.
Gutman E B, et al.
Cancer Research, 28(3), 485-495 (1936)
Kapil D Patel et al.
ACS applied bio materials, 2(11), 5190-5203 (2019-11-18)
Providing appropriate biophysical and biochemical cues to the interface is a facile strategy to enhance the osteogenic ability of metallic implants. Here we exploited this through the incorporation of mesoporous bioactive glass nanoparticles (MBGN) at a high content (1:1 by
Ruibin Kuang et al.
Plant physiology, 151(1), 199-209 (2009-07-28)
Purple acid phosphatase (PAP) catalyzes the hydrolysis of phosphate monoesters and anhydrides to release phosphate within an acidic pH range. Among the 29 PAP-like proteins in Arabidopsis (Arabidopsis thaliana), AtPAP15 (At3g07130) displays a greater degree of amino acid identity with
Gaurav Datta et al.
Frontiers in cellular neuroscience, 13, 329-329 (2019-08-06)
Human immunodeficiency virus type 1 (HIV-1) associated neuropathy is the most common neurological complication of HIV-1, with debilitating pain affecting the quality of life. HIV-1 gp120 plays an important role in the pathogenesis of HIV neuropathy via direct neurotoxic effects

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