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Key Documents

MAK447

Sigma-Aldrich

Alkaline Phosphatase Assay Kit

Sufficient for 250 colorimetric tests

Sinonimo/i:

ALP Activity Assay Kit, Alkaline Phosphatase Test Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

Livello qualitativo

applicazioni

pharmaceutical

Metodo di rivelazione

colorimetric

Malattie correlate

osteoporosis; gastrointestinal diseases; orthopedic diseases; cancer

Temperatura di conservazione

−20°C

Descrizione generale

Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis.

Applicazioni

The Alkalnine Phosphatase Assay Kit may be used for:
  • Cancer Research
  • Gastroenterology Disease Research
  • Osteoporosis Research

Caratteristiche e vantaggi

Enhanced Detection Range: Detect ALP levels accurately across a wide range, from 2 U/L to 800 U/L, using only 5 μL of sample in a 96-well plate setup, ensuring comprehensive analysis with minimal sample requirement.

Simplified Process: Experience a streamlined process with the addition of only a single working reagent and a short 5 minute assay reaction, reducing complexity and saving valuable time and effort.

Compatibility with High-Throughput Systems: Easily incorporate our kit into high-throughput handling systems, ensuring smooth and accurate processing, enhancing efficiency in your laboratory workflow.

Compatibilità

The kit is suitable for the quantitative determination of alkaline phosphatase (ALP) activity in serum, plasma etc.

Principio

The Alkaline Phosphatase Assay Kit is designed to measure ALP activity directly in biological samples without pretreatment. The improved method utilizes p-nitrophenyl phosphate that is hydrolyzed by ALP into a yellow colored product (maximal absorbance at A405 nm). The rate of the reaction is directly proportional to the enzyme activity.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Avvertenze

Danger

Classi di pericolo

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Organi bersaglio

Nervous system,Kidney,Liver,Skin

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials


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