MAK525

Acid Phosphatase Fluorometric Assay Kit

sufficient for 100 fluorometric tests

• Codice UNSPSC: 12161503
• NACRES: NA.84

Proprietà

• Livello qualitativo: 100
• Metodo di rivelazione: fluorometric
• Malattie correlate: genitourinary disorders; cancer; gastrointestinal diseases
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Acid Phosphatase (ACP) is an enzyme which catalyzes the cleavage of phosphate groups from other molecules during digestion. Acid phosphatase can be found in lysosomes and become active after fusing with endosomes, acidifying the pH and, thus, creating an optimal environment for ACP. ACP can also be found in bone, spleen, liver, kidney and blood. Serum levels can be used as a biomarker for prostatic carcinoma, although prostate-specific antigen (PSA) is more widely used.

Caratteristiche e vantaggi

• Linear detection range: 0.008 to 10 U/L .
• Compatible with high-throughput handling systems.

Compatibilità

The kit is suitable for acid phosphatase activity determination in biological samples (e.g. plasma, serum, cell lysate, tissue samples).

Principio

The Acid Phosphatase Fluorometric Assay Kit is based on the cleavage of a synthetic substrate. The product methylumbelliferone (MUB) becomes intensely fluorescent after addition of the stop reagent. The increase in fluorescence at λEx = 360 nm/λEm = 450 nm after addition of the stop reagent is directly proportional to the enzyme activity.

Informazioni sulla sicurezza

• Pittogrammi: GHS07
• Avvertenze: Warning
• Indicazioni di pericolo: H319
• Consigli di prudenza: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Classi di pericolo: Eye Irrit. 2
• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1