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M1255

Sigma-Aldrich

Calcein Blue

Powder

Sinonimo/i:

4-Methylumbelliferone-8-methyliminodiacetic acid

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About This Item

Formula empirica (notazione di Hill):
C15H15NO7
Numero CAS:
54375-47-2
Peso molecolare:
321.28
Numero CE:
259-129-8
Numero MDL:
MFCD00006867
Codice UNSPSC:
12171500
ID PubChem:
24896629
NACRES:
NA.47

product name

Calcein Blue, fluorescent dye

Livello qualitativo

200

Forma fisica

powder

tecniche

titration: suitable

Colore

white to light yellow

Solubilità

1 M NaOH: 50 mg/mL

Fluorescenza

λex 322 nm; λem 445 nm in methanol

Compatibilità

suitable for indicator for EDTA titration

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

CC1=CC(=O)Oc2c(CN(CC(O)=O)CC(O)=O)c(O)ccc12

InChI

1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
RJPSHDMGSVVHFA-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

Calcein Blue is a cell-permeable dye that stains viable cells. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. The staining applications of Calcein blue include tumor tissues, mineralized tissues, bone, amino acids, and many more.

Applicazioni

Calcein blue has been used fluorescent labeling of the mineralised sections of bone. Calcein blue AM ester hydrolyzed to calcein blue is suitable as a a viability stain, both microscopically, and in fluorescence activated cell sorting systems. It is also used as a merker of living cells like Schwann cells at neuromuscular junctions.

Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H315,H319,H335

Classi di pericolo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Respiratory system

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Indicators: International Series of Monographs in Analytical Chemistry (2013)
Juhyun Oh et al.
Aging, 8(11), 2871-2896 (2016-11-18)
Skeletal muscle is a highly regenerative tissue, but muscle repair potential is increasingly compromised with advancing age. In this study, we demonstrate that increased NF-κB activity in aged muscle fibers contributes to diminished myogenic potential of their associated satellite cells.
Zachary J Reitman et al.
Nature communications, 10(1), 3731-3731 (2019-08-21)
Pilocytic astrocytoma (PA), the most common childhood brain tumor, is a low-grade glioma with a single driver BRAF rearrangement. Here, we perform scRNAseq in six PAs using methods that enabled detection of the rearrangement. When compared to higher-grade gliomas, a
Tobias A Fuchs et al.
The Journal of cell biology, 176(2), 231-241 (2007-01-11)
Neutrophil extracellular traps (NETs) are extracellular structures composed of chromatin and granule proteins that bind and kill microorganisms. We show that upon stimulation, the nuclei of neutrophils lose their shape, and the eu- and heterochromatin homogenize. Later, the nuclear envelope
T Lymphocytes Influence the Mineralization Process of Bone.
El Khassawna T
Frontiers in Immunology, 8, 562-562 (2017)
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