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56496

Sigma-Aldrich

Calceina-AM

Small Package (20 X 50 μg ), ≥95.0% (HPLC)

Sinonimo/i:

Calceina O,O′-diacetato tetrakis(acetossimetil) estere

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About This Item

Formula empirica (notazione di Hill):
C46H46N2O23
Numero CAS:
148504-34-1
Peso molecolare:
994.86
Numero MDL:
MFCD05861516
Codice UNSPSC:
12171500
ID PubChem:
329758218
NACRES:
NA.32

Livello qualitativo

100

Saggio

≥95.0% (HPLC)

Fluorescenza

λex 496 nm; λem 516 nm±5 nm in 0.1 M Tris pH 8.0, esterase; Ca2+

Temperatura di conservazione

−20°C

Stringa SMILE

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)Cc1c(OC(C)=O)ccc2c1Oc3c(CN(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)c(OC(C)=O)ccc3C24OC(=O)c5ccccc45

InChI

1S/C46H46N2O23/c1-25(49)60-21-64-39(55)17-47(18-40(56)65-22-61-26(2)50)15-32-37(68-29(5)53)13-11-35-43(32)70-44-33(16-48(19-41(57)66-23-62-27(3)51)20-42(58)67-24-63-28(4)52)38(69-30(6)54)14-12-36(44)46(35)34-10-8-7-9-31(34)45(59)71-46/h7-14H,15-24H2,1-6H3
XKFSBWQWNMZWFA-UHFFFAOYSA-N

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Descrizione generale

Calcein-AM, also known as Calcein-acetoxymethyl ester, is a non-fluorescent molecule converted into an anionic fluorescent form by intracellular esterase enzymes. It provides a continuous visualization of adherent cells during the experiment. Calcein-AM is a vital dye that introduces Calcein in living cells with intact cell membranes. The fluorescent part of Calcein-AM localizes in the intracellular spaces after esterase-dependent cellular trapping, displaying cytotoxic activity in cells.

Applicazioni

Calcein-AM is used as a cell viability stain and as a neutral substrate for multidrug (MDR) efflux transporters. In cancer research, it is used as a neutral substrate for multidrug resistance protein MRP. Fluorescence assays to determine human erythrocyte viability and aging uses Calcein-AM as a probe.
Derivato della calceina permeabile alle cellule, diventa fluorescente per idrolisi. Usato come substrato neutro per la proteina MRP resistente a più farmaci impiegata nella ricerca dei tumori.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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