E1903

Ethidium homodimer I solution

≥95% purity (HPLC), liquid

• Sinonimo/i: EtDi, EthD-1, Phenanthridinium, 5,5′-[1,2-ethanediylbis(imino-3,1-propanediyl)]bis(3, 8-diamino-6-phenyl)-,dichloride,dihydrochloride
• Formula empirica (notazione di Hill): C₄₆H₅₀Cl₄N₈
• Peso molecolare: 856.75
• Numero CE: 200-664-3
• Numero MDL: MFCD00210948
• Codice UNSPSC: 12171500
• ID PubChem: 24894444
• NACRES: NA.47

Proprietà

• product name: Ethidium homodimer I solution, ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC), 2 mM in DMSO
• Livello qualitativo: 100
• Saggio: ≥95% (Run on Ethidium Homodimer before dissolution in DMSO, TLC)
• Forma fisica: liquid
• Concentrazione: 2 mM in DMSO
• tecniche: titration: suitable
• Colore: red
• ε (coefficiente d’estinzione): ≥9000 at 490-496 nm in water
• applicazioni: diagnostic assay manufacturing; hematology; histology
• Temperatura di conservazione: −20°C
• Stringa SMILE: Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28
• InChI: 1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H; ZKAJUKABUUJRLB-UHFFFAOYSA-N

Descrizione

Applicazioni

Ethidium homodimer I solution has been used to check membrane integrity in sperms.

Stains ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA structures.

Used to detect DNA in solution.

Azioni biochim/fisiol

Ethidium homodimer is a red fluorescent dye. It cannot cross an intact plasma membrane, but can easily pass through a damaged plasma membrane. Ethidium homodimer is used to check membrane integrity.

One molecule of EthD-1 will non-selectively bind four base pairs in dsDNA.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): 185.0 °F - closed cup
• Punto d’infiammabilità (°C): 85 °C - closed cup
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)