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Merck
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Documenti fondamentali

03285

Sigma-Aldrich

Cell Counting Kit

sufficient for 500 tests

Sinonimo/i:

Fluorescent Cell Counter

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
12352116
eCl@ss:
32161000
NACRES:
NA.32

Stato

liquid

impiego

sufficient for 500 tests

Temperatura di conservazione

−20°C

Descrizione generale

Cell Counting kit, also known as FCCK, is a fluorescent cell counting product. The amount of a fluorescent dye, calcein, produced from Calcein-AM (3′,6′-Di(O-acetyl)-2′,7′-bis [N, Nbis-(carboxymethyl) aminomethyl]-fluorescein, tetraacetoxymethyl ester) by esterases in cells is directly proportional to the number of viable cells in a culture medium. Calcein-AM is highly lipophilic due to the presence of acetoxymethyl groups in the molecule, which rapidly permeates into the cell cytoplasm through the membrane.

Applicazioni

Cell Counting Kit or FCCK is used for the detection of live cells by fluorometric method.

Caratteristiche e vantaggi

Cell Counting Kit has the following advantages:

  • It doesn’t require any radioisotopes to function.
  • No solubilization procedure is needed.
  • Results are highly reproducible and accurate.

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Articoli

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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