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L7018

Sigma-Aldrich

Lipopolysaccharides from Pseudomonas aeruginosa 10

purified by trichloroacetic acid extraction

Sinonimo/i:

LPS

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About This Item

Numero MDL:
Codice UNSPSC:
12352201
NACRES:
NA.25

Origine biologica

Pseudomonas aeruginosa (10)

Livello qualitativo

Stato

lyophilized powder

Purificato mediante

trichloroacetic acid extraction

Impurezze

1-10% Protein (Lowry)

Colore

white to yellow

Solubilità

water: 4.90-5.10 mg/mL, hazy to turbid, colorless to light yellow

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

This product is TCA extracted from Pseudomonas aeruginosa serotype 10. The source strain is ATCC 27316.

Applicazioni

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Azioni biochim/fisiol

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota sulla preparazione

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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