L3397

LB Broth (Luria low salt)

Powder microbial growth medium

• Sinonimo/i: Luria Broth (Miller’s Modification), Luria-Bertani medium (LB)
• Codice UNSPSC: 41106200
• NACRES: NA.85

Proprietà

• Grado: for molecular biology
• Livello qualitativo: 200
• Sterilità: non-sterile
• Stato: powder
• Composizione: NaCl, 0.5 g/L ; Tryptone, 10 g/L ; Yeast Extract, 5 g/L
• tecniche: microbiological culture: suitable
• pH: 6.8-7.2(1.55% solution)
• Compatibilità: suitable for molecular biology; nonselective for Escherichia coli; nonselective for coliforms
• applicazioni: food and beverages; microbiology
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

Luria Broth is a bacterial culture medium used for the cultivation of members of the Enterobacteriaceae especially E.coli, as well as for coliphage plaque assays. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a very low-salt formulation.

Applicazioni

LB Broth (Luria low salt) has been used as a liquid broth medium in the broth microdilution technique.

Caratteristiche e vantaggi

Luria′s low salt LB powder provides:

• Standard formulation
• A budget-friendly alternative to liquid
• Easy scale-up using larger package sizes

Nota sulla preparazione

1. Suspend 15.5 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121°C.

Ricostituzione

Stir to suspend 15.5g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable