CMC0014
BL21(DE3) Chemically Competent Cells
Escherichia coli, rod shaped
Sinonimo/i:
Competent E. coli cells
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About This Item
Codice UNSPSC:
41106202
NACRES:
NA.85
Prodotti consigliati
Nome del prodotto
BL21(DE3) Chemically Competent Cells, for protein expression
Origine biologica
Escherichia coli
Grado
for molecular biology
Modalità di accrescimento
adherent or suspension
Morfologia
rod shaped
tecniche
microbiological culture: suitable
Trasformazione cellulare
competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Categorie correlate
Descrizione generale
The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Genotype
F – ompT hsdSB (rB- mB-) gal dcm (DE3)
Applicazioni
The E. coli strain BL21 (DE3) has been used:
- to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
- BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
- to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.
Caratteristiche e vantaggi
- a transformation efficiency of >1 × 107 cfu/μg.
- economical prices
- offering superior value for everyday protein expression work.
Componenti
- BL21(DE3) chemically competent cells
- pUC 19 transformation control DNA
- recovery medium for expression
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
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