Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

View All Documentation

L3272

Sigma-Aldrich

LB Broth with agar (Luria low salt)

Powder microbial growth medium

Sinonimo/i:

Luria Agar Base (Miller’s Modification)

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Codice UNSPSC:
41106200
NACRES:
NA.85

Grado

for molecular biology

Livello qualitativo

200

Sterilità

non-sterile

Forma fisica

powder

Composizione

Agar, 15 g/L
NaCl, 0.5 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L

tecniche

microbiological culture: suitable

pH

6.8-7.2(3.05% solution)

applicazioni

food and beverages
microbiology

Temperatura di conservazione

room temp

Compatibilità

nonselective for Escherichia coli
nonselective for coliforms

Descrizione generale

Luria′s low salt LB is a microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates. The addition of agar provides a solid medium for microbial growth.

Applicazioni

LB Broth with agar (Luria low salt) has been used:
  • for culturing E. coli DH10B cells.
  • in the isolation of diazotrophic bacteria.
  • for transformation of competent E. coli strain Stbl3 for cloning.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin®.

Caratteristiche e vantaggi

Luria′s low salt LB powder with agar provides:
  • Easy scale-up using larger package sizes
  • A budget-friendly alternative to pre-poured plates
  • Standard formulation

Nota sulla preparazione

1. Suspend 30.5 g in 1 L of distilled water.
2. Heat to boiling while stirring to dissolve.
3. Autoclave for 15 minutes at 121°C.
4. Cool to 50°C prior to dispensing into petri dishes.

Note legali

Zeocin is a registered trademark of Cayla Sarl

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 8

1 of 8

LB Broth with agar (Lennox) Tablet microbial growth medium

Sigma-Aldrich

L7025

LB Broth with agar (Lennox)

Brodo LB (Lennox) Highly-referenced microbial growth powder medium, low salt, suitable for salt-sensitive E.coli culture.

Sigma-Aldrich

L3022

Brodo LB (Lennox)

LB Broth (Miller) Liquid microbial growth medium

Sigma-Aldrich

L2542

LB Broth (Miller)

LB broth, Miller Microbiologically tested

Supelco

L3152

LB broth, Miller

LB Broth (Lennox) Tablet microbial growth medium

Sigma-Aldrich

L7275

LB Broth (Lennox)

LB Broth NutriSelect® Plus, from non-animal source(Vegitone), powder, pkg of 500 g, non-sterile, nonselective for Escherichia coli

Millipore

28713

LB Broth

NZCYM Broth Powder microbial growth medium

Sigma-Aldrich

N3643

NZCYM Broth

LB Broth GranuCult® plus, granular, pack of 500 g or 5 kg, for Escherichia coli

Millipore

1.10285

LB Broth

Difco Manual, 271-271 (1998)
Increased Expression of a Reporter Plasmid by Crotalid Venom Fraction
Islas AA, et al.
Journal of Animal and Veterinary Advances, 9(17), 2214-2219 (2010)
Using plant growth-promoting rhizobacteria (PGPR) to improve plant growth
Grobelak A, et al.
Ecological Engineering, 84, 22-28 (2015)
Miller, J.H.
Experiments in Molecular Genetics, 433-433 (1972)
Rupal Mistry et al.
Genetics, 206(2), 889-904 (2017-04-18)
Resident gut bacteria are constantly influencing the immune system, yet the role of the immune system in shaping microbiota composition during an organism's life span has remained unclear. Experiments in mice have been inconclusive due to differences in husbandry schemes
Visualizza tutti i documenti correlati

Protocolli

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.