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I2911

Sigma-Aldrich

IMDM

StableCell™, with stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

IMDM

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About This Item

Numero MDL:
MFCD00217469
Codice UNSPSC:
12352207
NACRES:
NA.75

product name

Terreno Dulbecco modificato di Iscove, With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Livello qualitativo

400

Sterilità

sterile-filtered

Forma fisica

liquid

tecniche

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Impurezze

Endotoxin, tested

Componenti

NaHCO3: yes
glucose: yes
stable glutamine: yes
phenol red: yes

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.

Applicazioni

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Note legali

StableCell is a trademark of Sigma-Aldrich Co. LLC

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Proceedings of the National Academy of Sciences of the United States of America, 105(25), 8691-8696 (2008-06-20)
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Recent advances in cell biology and gene regulation suggest mechanisms whereby associative learning could be performed by single cells. Therefore, we explored a model of classical conditioning in human macrophages in vitro. In macrophage cultures, bacterial lipopolysaccharide (LPS; unconditioned stimulus)
Florian A Salomons et al.
Molecular and cellular biology, 29(7), 1774-1785 (2009-01-23)
Conditions causing an increase in misfolded or aberrant proteins can impair the activity of the ubiquitin/proteasome system (UPS). This observation is of particular interest, given the fact that proteotoxic stress is closely associated with a large variety of disorders. Although

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