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I7633

Sigma-Aldrich

IMDM

With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture

Sinonimo/i:

IMDM

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About This Item

Numero MDL:
MFCD00217469
Codice UNSPSC:
12352207
NACRES:
NA.75

product name

Terreno Dulbecco modificato di Iscove, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Forma fisica

powder

tecniche

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

Componenti

phenol red: yes
glucose: yes
L-glutamine: yes

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Applicazioni

Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line) and Chinese hamster ovary (CHO) DG44 cell line.
Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Quantità

Formulated to contain 17.7 grams of powder per liter of medium.

Ricostituzione

Supplement with 3.024 g/L sodium bicarbonate.

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Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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