M4892
McCoy′s 5A Medium
Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Produzione farmaceutica
Sinonimo/i:
5A basal medium
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About This Item
Prodotti consigliati
Livello qualitativo
Stato
powder
tecniche
cell culture | mammalian: suitable
Componenti
phenol red: 0.011 g/L
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
NaHCO3: no
Condizioni di spedizione
ambient
Temperatura di conservazione
2-8°C
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Descrizione generale
McCoy′s 5A Medium is recommended to produce viruses in primary cell cultures.
Applicazioni
McCoy′s 5A Medium has been used for culturing:
- human colon carcinoma cell lines (HCT116, RKO, FET, CBS, HCT116b, and TENN) to study the function of leucine-rich repeat-containing G-protein-coupled receptor (LGR5) in colon cancer metastasis
- human colorectal HCT116 cells to study miR-224 over-expression in cancer
- osteoblasts to study ion reactivity of calcium-deficient hydroxyapatite in standard cell culture media
Azioni biochim/fisiol
McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.
Quantità
Formulated to contain 11.9 grams of powder per liter of medium.
Ricostituzione
Supplement with 2.2 g/L sodium bicarbonate.
Comunemente ordinati con questo prodotto
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
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Protocolli
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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