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Key Documents

I2393

Sigma-Aldrich

Interleukin-1β from rat

IL-1β, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Sinonimo/i:

IL-1β, Interleukin-1β from rat, Lymphocyte activating factor

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About This Item

Numero MDL:
Codice UNSPSC:
12352202

Origine biologica

rat

Livello qualitativo

Ricombinante

expressed in E. coli

Saggio

≥98% (SDS-PAGE and HPLC)

Forma fisica

lyophilized powder

Potenza

0.01-0.1 ng/mL ED50/EC50

PM

17.3 kDa

Confezionamento

pkg of 10 and 50 μg

tecniche

cell culture | mammalian: suitable

Impurezze

<0.1 EU/μg

N° accesso UniProt

Temperatura di conservazione

−20°C

Informazioni sul gene

rat ... Il1b(24494)

Descrizione generale

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cells and lymphocytes, which then proliferate and secrete interleukin-2.1 IL-1 is primarily released from stimulated macrophages and monocytes, but is also released from several other cell types,2 and is thought to play a key role in inflammatory and immune responses.3 Other synonyms for IL-1 include: Endogenous Pyrogen (EP), Mitogenic Protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFH), B cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF).4 The two closely related agents, Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) bind to the same cell surface receptor, elicit nearly identical biological responses and yet share 25% homology in their amino acid sequence. Recombinant rat IL-1β is a 17.3 kDa protein containing 153 amino acid residues.

Stato fisico

Lyophilized from a 0.2 μm filtered buffered solution.

Risultati analitici

The biological activity is measured in a cell proliferation assay using murine D10S cells.

Citazione

1. Gery, I., et al., J. Exp. Med., 136, 128 (1972).
2. Oppenheim, J. J., et al., Immunol. Today, 7, 45 (1986).
3. Durum, S. K., et al., Annu. Rev. Immunol., 3, 263 (1985).
4. Aarden, L., et al., J. Immunol., 123, 2928 (1979).
5. Symons, J., et al., Lymphokines and Interferons, A Practical Approach, Clemens, M., et al., (eds.), IRL Press, Oxford (1987).

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Articoli

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages and monocytes and plays a key role in inflammatory and immune responses and may induce anti-tumor immunity.

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