H7666

IL-12 human

HumanKine^®, recombinant, Xeno-free, >95% (SDS-PAGE), suitable for cell culture

• Sinonimo/i: IL-12
• Numero MDL: MFCD00212975
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• product name: Interleukin-12 human, HumanKine^®, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin, tested
• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in HEK 293 cells
• Saggio: ≥95% (SDS-PAGE)
• Forma fisica: lyophilized
• Potenza: ≤2 ng/mL EC₅₀
• PM: dimer 57 kDa (glycosylated)
• Confezionamento: pkg of 10 μg
• tecniche: cell culture | mammalian: suitable
• Impurezze: endotoxin, tested
• Temperatura di conservazione: −20°C

Descrizione

Applicazioni

Interleukin-12 (IL-12) human has been used to study IL-12 mediated enhancement of IL-12Rβ2 (IL-12 receptor) expression.

Azioni biochim/fisiol

Interleukin-12 (IL-12) was identified as a factor secreted by human Epstein-Barr (EBV)-transformed B cell lines. IL-12, or Natural Killer Cell Stimulatory Factor (NKSF), is a 75 kDa disulfide-linked heterodimer of a 35 kDa subunit and 40 kDa subunit. IL-12 is produced predominantly by monocytes and NK cells and induces T cells and NK cells to produce IFN-γ. Human IL-12 is not active on mouse cells, but murine IL-12 is active on both murine and human lymphocytes. The biological activity of recombinant human IL-12 was measured by its ability to stimulate the proliferation of PHA-activated human T lymphoblasts. IL-12 has anti-angiogenic properties.

Stato fisico

lyophilized from a 0.2 μm filtered solution of PBS

Risultati analitici

The specific activity was determined by the dose-dependent release of IFN-γ from the human NK92 cell line in presence of 20 ng/mL rIL-2.

Note legali

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable