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H0164

Sigma-Aldrich

Anti-Histone H3 antibody produced in rabbit

enhanced validation

IgG fraction of antiserum, buffered aqueous solution

Sinonimo/i:

Anti H3 Antibody, Histone H3 Antibody, Histone H3 Antibody - Anti-Histone H3 antibody produced in rabbit

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

IgG fraction of antiserum

Tipo di anticorpo

primary antibodies

Clone

polyclonal

Forma fisica

buffered aqueous solution

PM

antigen 17 kDa

Reattività contro le specie

chicken, mouse, plant, Drosophila, rat, human, Xenopus

Confezionamento

antibody small pack of 25 μL

Convalida avanzata

independent
Learn more about Antibody Enhanced Validation

tecniche

indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cells
western blot: 1:2,500-1:5,000 using whole cell extract of the rat pheochromocytoma PC12 cell line
western blot: 1:5,000-1:10,000 using whole cell extract of the A431 human epidermoid carcinoma cell line or a whole cell extract of the mouse fibroblast NIH3T3 cell line

N° accesso UniProt

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Histone proteins are known to stabilize and regulate the nuclear DNA. Core histones, H2A, H2B, H3 and H4 aid the packaging of eukaryotic nuclear DNA into repeating nucleosome units while the linker histone, H1, binds to DNA between the nucleosomes. A diverse and elaborate array of post-translational modifications, including acetylation, phosphorylation and methylation, ubiquitination and ADP ribosylation occur in histones, particularly on the N-terminal tail domains of histones, particularly of H3 and H4.These modifications dictate chromatin states and the gene expression.
Phosphorylation of H3, mainly on Ser10 and Ser28, is localized to a small fraction of highly acetylated H3 and occurs primarily in response to mitogenic and stress stimuli. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Histone methylation, like acetylation, is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis ad heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9, and Lys27 are the preferred sites of methylation. Histone lysine residues can be mono-, di-, and tri-methylated at different heterochromatic subdomains, adding further complexity to the regulation of chromatin structure.

Specificità

Anti-Histone H3 specifically recognizes histone H3.

Immunogeno

synthetic peptide corresponding to amino acids 125-136 located at the C-terminus of human histone H3, conjugated to KLH. This sequence is identical in many species including rat, mouse, chicken, Xenopus, Drosophila, and plant histone H3.

Applicazioni

Anti-Histone H3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Prodotti correlati

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificati d'analisi (COA)

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Histone H3 is absent from organelle nucleoids in BY-2 cultured tobacco
cells
Takusagawa M
Cell Biology International, 37, 748-754 (2013)
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Singh P
Journal of Neurochemistry, 134, 642-651 (2015)
Evidence that core histone H3 is targeted to the mitochondria in Brassica oleracea mitochondria in Brassica oleracea
Katherine B Zanin M et al
Cell Biology International, 34, 997-1003 (2012)
MAP kinase-mediated signalling to nucleosomes and immediate-early gene induction.
Thomson S et al
Seminars in Cell Biology, 10, 205-214 (1999)
M J Barratt et al.
Proceedings of the National Academy of Sciences of the United States of America, 91(11), 4781-4785 (1994-05-24)
Diverse agents, including growth factors and phorbol esters, induce rapid transcriptional activation of a subset of immediate-early (IE) genes that include the protooncogenes c-fos and c-jun. Among the earliest nuclear signaling events concomitant with IE gene activation is the phosphorylation

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