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Key Documents

GE28-9321-71

HIS Spintrap™ Kit

Cytiva 28-9321-71, pack of 1 kit

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

Confezionamento

pack of 1 kit

Produttore/marchio commerciale

Cytiva 28-9321-71

Condizioni di stoccaggio

(0.15% Kathon CG)
 (20% Ethanol)

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-14(Ni2+-stripped medium.)

working range

3-12(Ni2+-stripped medium.)

Capacità

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

His SpinTrap is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Caratteristiche e vantaggi

  • For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
  • Ni Sepharose High Performance in prepacked spin columns.
  • Yields up to 750 µg pure protein per column.
  • Allows direct purification of both clarified and unclarified cell lysates.

Componenti

The kit contains:

1 pack - GE28-4013-53 - His Spin Trap™
1 pack - GE11-0034-00 - His Buffer Kit

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

SpinTrap is a trademark of Cytiva

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • GE11-0034-00His Buffer Kit, Cytiva 11-0034-00SDS
  • GE28-4013-53His Spintrap™, Cytiva 28-4013-53SDS

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H314

Codice della classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Optimizing Purification of Histidine-Tagged Proteins

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

Protocolli

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from GE Healthcare.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.

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