GE11-0033-99

His GraviTrap^™

Cytiva 11-0033-99

• Sinonimo/i: His GraviTrap Column
• Codice UNSPSC: 12161501
• NACRES: NA.32

Proprietà

• Confezionamento: pkg of 10 ea
• Produttore/marchio commerciale: Cytiva 11-0033-99
• Volume del letto: 1 mL
• Diam. int. colonna: 15 mm
• Matrice: 6% cross-linked agarose
• Dimensione particelle: 45-165 μm
• Diametro medio: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium)
• working range: 3-12(Ni²⁺-stripped medium.)
• Capacità: ~40 mg binding capacity (histidine-tagged protein/column)(Protein binding capacity is protein-to-protein dependent.); ~40 mg binding capacity(histidine-tagged protein)
• Compatibilità: suitable for bioprocess medium

Descrizione

Descrizione generale

His GraviTrap^™ is a prepacked, single-use column for purification of histidine-tagged proteins by immobilized metal affinity chromatography (IMAC). Column gives fast and simple gravity-flow purifications without any need for a purification.

The ready to use column is prepacked with Ni Sepharose^™ 6 Fast Flow, which has a high protein binding capacity, low nickel-ion leakage, and is compatible with denaturing and reducing agents, as well a wide range of additives.

His GraviTrap^™ columns are delivered in a package that can be converted into a column stand to simplify purification. LabMate PD-10 Buffer Reservoir can be connected to the columns for convenient handling of sample volumes above 10 mL.

Caratteristiche e vantaggi

• For fast and simple purification of histidine-tagged* proteins.
• Convenient, prepacked gravity-flow column.
• Allows direct purification of both clarified and unclarified cell lysates.
• High binding capacity; approximately 40 mg of pure protein per column.
• Compatible with a wide range of reducing agents, detergents, denaturants and other additives.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

GraviTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS09
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Codice della classe di stoccaggio: 3 - Flammable liquids