GE28-4013-53

His Spintrap^™

Cytiva 28-4013-53

• Sinonimo/i: His Spintrap Column
• Codice UNSPSC: 41106500
• NACRES: NA.32

Proprietà

• Produttore/marchio commerciale: Cytiva 28-4013-53
• Confezionamento: pkg of 50 x 100 μL
• Volume del letto: 100 μL
• Matrice: highly cross-linked 6% agarose
• Dimens. media delle particelle: 34 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• Capacità: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Descrizione

Descrizione generale

His SpinTrap^™ is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)

* See the section for licensing information in our terms and conditions.

Purification of unclarified samples on His SpinTrap^™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap^™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.

His SpinTrap^™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap^™ together with buffers prepared using His Buffer Kit.

The columns are prepacked with Ni Sepharose^™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose^™ High Performance medium is negligible.

Caratteristiche e vantaggi

• For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
• Ni Sepharose^™ High Performance in prepacked spin columns.
• Yields up to 750 μg pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.15% Kathon CG)

Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS09
• Avvertenze: Warning
• Indicazioni di pericolo: H317
• Consigli di prudenza: P280 - P261 - P272 - P302 + P352 - P333 + P313 - P501
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids