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Key Documents

GE28-9031-35

Protein G High Performance MultiTrap

Cytiva 28-9031-35

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About This Item

Codice UNSPSC:
41105323
NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

Materiali

polypropylene and polyethylene

Caratteristiche

wetted part: no

Confezionamento

pkg of 4 ea

Produttore/marchio commerciale

Cytiva 28-9031-35

Condizioni di stoccaggio

(20% Ehtanol)
(20% Ethanol)

Parametri

30 °C max. temp.
4 °C min. temp.

Misura

127.8 mm × 85.5 mm × 30.6 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-9

working range

3-9

Capacità

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)

Temperatura di conservazione

2-8°C

Descrizione generale

Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species.

Protein G Sepharose HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).

A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caratteristiche e vantaggi

  • Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
  • Flexible format, protocol for classical elution as well as cross-linking method.
  • Formatted for elution for electrophoresis or LC-MS analysis workflows.
  • Fast binding kinetics and high capacity provides high yield.
  • Easy scale-up with HiTrap® Protein G HP prepacked columns.
  • Prepacked with Protein G Sepharose High Performance for coupling of antibodies of IgG subclasses.
  • MultiTrap 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

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Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

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