Passa al contenuto
Merck
Tutte le immagini(3)

Documenti fondamentali

GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

Sinonimo/i:

HiTrap Protein G, Protein G affinity column

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(3)

About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

Stato

prepacked column

Crosslinking

cross-linked

Confezionamento

pack of 2 × 1 mL

Produttore/marchio commerciale

Cytiva 17-0404-03

Condizioni di stoccaggio

(20% Ehtanol)
 (20% Ethanol)

Parametri

42 psi

tecniche

liquid chromatography (LC): suitable

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Misura

Matrice

(cross-linked agarose, spherical)

Gruppo funzionale matrice

Protein G

Dimens. media delle particelle

34 μm

cleaning

2-9

Intervallo di esercizio

3-9

Capacità

>1 mg binding capacity (lgG/column)
 25 mg binding capacity(human IgG/column)

Tecnica di separazione

affinity

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose™ High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caratteristiche e vantaggi

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documenti section.

Se ti serve aiuto, non esitare a contattarci Servizio Clienti

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

Articoli

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

This page describes automated purification of antibodies using ÄKTA chromatography systems.

Performing a separation with HiTrap Protein G HP

This page shows how to perform a separation with the HiTrap Protein G HP from GE Healthcare, an Affinity Exclusion Chromatography product containing pre-packed Protein G.

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

Vedi tutto

Protocolli

Purification of Mouse Monoclonal IgG1 using HiTrap Protein

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

General Instructions for Affinity Purification Using HiTrap® Columns

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.