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Key Documents

GE28-4017-45

GSTrap 4B

Cytiva 28-4017-45, pack of 5 × 1 mL

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

glutathione

Forma fisica

prepacked column

Crosslinking

cross-linked

Confezionamento

pack of 5 × 1 mL

Produttore/marchio commerciale

Cytiva 28-4017-45

Condizioni di stoccaggio

(20% Ethanol)

Parametri

<4 mL/min flow rate
42 psi (H2O at 25°C)

tecniche

liquid chromatography (LC): suitable

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

7 mm

Matrice

4% agarose

Gruppo funzionale matrice

glutathione functional group

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning

4-13

working range

4-13

Capacità

>25 mg binding capacity (horse liver GST/ml medium)

Tecnica di separazione

affinity

Descrizione generale

GSTrap 4B columns are prepacked Glutathione Sepharose 4B columns for convenient, high capacity one-step purification of glutathione S-transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Applicazioni

GST, see the section for licensing information in our terms and conditions, tagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scale-up. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA® design or FPLC System.

Caratteristiche e vantaggi

  • High binding capacity.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Easy one-step purifications of Glutathione S-Transferase (GST) tagged Proteins resulting in high purity.
  • GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA® design.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

FPLC is a trademark of Cytiva
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

Vedi tutto

Protocolli

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

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