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Key Documents

GE17-5282-01

GSTrap High Performance

Cytiva 17-5282-01, pack of 1 × 5 mL

Sinonimo/i:

GSTrap Column

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

glutathione

Confezionamento

pack of 1 × 5 mL

Produttore/marchio commerciale

Cytiva 17-5282-01

Parametri

flow rate
<20 mL/min flow rate
42 psi (H2O at room temperature.)

Dimensioni del letto

16 mm × 25 mm

Volume del letto

5 mL

Diam. int. colonna

16 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

3-12

working range

3-12

Capacità

>7 mg/mL binding capacity (medium)

Descrizione generale

GSTrap HP columns are prepacked with Glutathione Sepharose High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Applicazioni

GSTrap HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged Proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose High Performance. GST-tagged Proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.

GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.

Caratteristiche e vantaggi

  • High resolution purification of GST-tagged Proteins.
  • Mild elution conditions preserving Protein antigenicity and function.
  • Fast, one-step purification.
  • Easy to use with syringe, pump or a chromatography system.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from GE Healthcare.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

Vedi tutto

Protocolli

This page describes how to purify GST-tagged proteins using GST HiTrap 1 ml and 5 ml columns: GSTrap 4B, GSTrap FF and GSTrap HP from GE Healthcare.

This page shows how to purify GST-tagged proteins using GSTrap HP, GSTrap FF and GSTrap 4B columns from GE Healthcare.

Characteristics of Glutathione Sepharose® Products

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