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GE17-0756-01

Glutathione Sepharose™ 4B

Cytiva 17-0756-01, pack of 10 mL

Sinonimo/i:

affinity chromatography resin, GST-tagged proteins purification,

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione

Confezionamento

pack of 10 mL

Produttore/marchio commerciale

Cytiva 17-0756-01

Matrice

4% agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning in place

4-13

Intervallo di esercizio

4-13

Capacità

>25 mg binding capacity (horse liver GST/ml medium)

Categorie correlate

Descrizione generale

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Caratteristiche e vantaggi

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

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Certificati d'analisi (COA)

Lot/Batch Number

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Articoli

Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins

This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.

Purification Using GST SpinTrap

This page shows how to purify GST-tagged proteins using GST SpinTrap from GE Healthcare.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Vedi tutto

Protocolli

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

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Global Trade Item Number

SKUGTIN
GE17-0756-014061837620669

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