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Key Documents

GE17-0756-01

Glutathione Sepharose™ 4B

Cytiva 17-0756-01, pack of 10 mL

Sinonimo/i:

affinity chromatography resin, GST-tagged proteins purification,

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

glutathione

Confezionamento

pack of 10 mL

Produttore/marchio commerciale

Cytiva 17-0756-01

Matrice

4% agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning in place

4-13

working range

4-13

Capacità

>25 mg binding capacity (horse liver GST/ml medium)

Categorie correlate

Descrizione generale

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

GST-tagged proteins and other glutathione S-transferases or glutathone-dependent proteins can be purified directly from bacterial lysates using Glutathione Sepharose™ 4B. Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity.

Caratteristiche e vantaggi

  • High capacity single step purification of recombinant glutathione S-transferase (GST) tagged proteins
  • Proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and functionality.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Troubleshooting of Problems Associated with the Expression and Growth of GST-tagged Proteins

This page addresses common problems associated with the expression and growth of GST-tagged proteins using GE Healthcare products.

Purification Using GST SpinTrap

This page shows how to purify GST-tagged proteins using GST SpinTrap from GE Healthcare.

General Considerations for Purification of GST-tagged Proteins

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Vedi tutto

Protocolli

GST Detection Module with CDNB Enzymatic Assay

This page describes enzymatic detection of GST-tagged proteins with the GST Detection Module from GE Healthcare.

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from GE Healthcare.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Purification Using GST HiTrap 1 mL and 5 mL Columns: GSTrap 4B, GSTrap FF, and GSTrap HP

This page describes how to purify GST-tagged proteins using GST HiTrap 1 ml and 5 ml columns: GSTrap 4B, GSTrap FF and GSTrap HP from GE Healthcare.

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