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GE17-5110-01

HiTrap® IGM purification

Cytiva 17-5110-01, pack of 5 × 1 mL

Sinonimo/i:

IGM Purification Column

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

Confezionamento

pack of 5 × 1 mL

Produttore/marchio commerciale

Cytiva 17-5110-01

Condizioni di stoccaggio

(20% Ethanol)

Parametri

<4 mL/min flow rate
42 psi

Dimensioni del letto

7 mm × 25 mm

Volume del letto

1 mL

Diam. int. colonna

16 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-13

Intervallo di esercizio

3-11

Temperatura di conservazione

2-8°C

Descrizione generale

HiTrap® IgM Purification HP is a 1 mL column prepacked with Sepharose™ High Performancefor fast and efficient purification of monoclonal IgM.

Applicazioni

HiTrap® IgM Purification HP is prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgM and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Caratteristiche e vantaggi

  • Optimized for fast, efficient purification of monoclonal IgM from hybridoma cell culture.
  • Prepacked with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose™ High Performance.
  • Recovery of IgM is high with excellent activity retention.
  • The top and bottom frits are manufactured from porous polyethylene
  • Columns are delivered with a stopper on the inlet and a snap-off end on the outle

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

Prodotti correlati

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Descrizione
Determinazione del prezzo

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

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Certificati d'analisi (COA)

Lot/Batch Number

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Articoli

Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolli

HIC Purification Options and Scale up with SOURCE™ Media

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

Buffer Exchange and Desalting for Affinity Chromatography

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

Contenuto correlato

Performing a Separation with HiTrap® IgM Purifcation HP

How to perform a separation with the HiTrap IgM Purifcation HP from GE Healthcare which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.

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