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Key Documents

GE17-5111-01

HiTrap® IGY Purification

Cytiva 17-5111-01, pack of 1 × 5 mL

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

2-mercaptopyridine

Durata

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Confezionamento

pack of 1 × 5 mL

Produttore/marchio commerciale

Cytiva 17-5111-01

Condizioni di stoccaggio

(20% Ethanol)

Parametri

<20 mL/min flow rate (The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.)
42 psi

Dimensioni del letto

16 mm × 25 mm

Volume del letto

5 mL

Diam. int. colonna

16 mm

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-13

working range

3-11

Temperatura di conservazione

2-30°C

Categorie correlate

Descrizione generale

HiTrap® IgY Purification HP is a 5 mL column prepacked with Sepharose High Performance for fast and easy purification of IgY from egg yolk.

Applicazioni

HiTrap® IgY Purification HP is packed with a thiophilic adsorption medium, 2-merCaptopyridine coupled to Sepharose High Performance. Thiophilic adsorption is promoted by water-structuring salts. It has been suggested that the interaction of IgY and ligand results from combined electron donating and accepting action of the ligand or, alternatively, a mixed mode hydrophilic-hydrophobic interaction.

Caratteristiche e vantaggi

  • Each column binds IgY from a one-quarter portion of egg yolk (approx. 100 mg IgY). For greater capacity, several columns may be connected in series.
  • Packed with a thiophilic adsorption medium, the ligand, 2-merCaptopyridine is coupled to Sepharose High Performance.
  • Recovery and purity of IgY are excellent.
  • Can be operated with a liquid chromatography system such as ÄKTA® design.
  • The top and bottom frits are manufactured from porous polyethylene
  • Connectors for usage with different chromatography systems and other equipment are included

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Pittogrammi

Flame

Avvertenze

Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

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Protocolli

This page shows how to prepare samples for purification with affinity chromatography.

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